Endpoint comparison of CG1, CG3 and CG9 animals with their respective DIO control groups at 8, 9.5 and 12 months respectively. (A) Subcutaneous adipocyte (SAT) number and (B) size. Data is from two representative male fish of the cohort; (C) CG1-9 fish were compared for whole body adiposity with DIO fish using μCT imaging; (D-E) quantification of visceral (VAT) and subcutaneous (SAT) adipose tissue by μCT, data from 4–5 mixed gender fish. (F) representative hepatic histology of the CG group stained with hematoxylin and oil red O (ORO). Sections show little ORO staining and few lesions (compare with Fig 4F). Error bars indicate STDEV, groups were compared with an ANOVA followed by Šídák’s multiple comparisons test, significance is indicated as * p<0.05, ** p<0.01 and *** p<0.001.

Acknowledgments

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