Fig. 3

Fig. 3

Selective breeding-induced and mirror-induced neurotranscriptomic differences of male and female high aggression zebrafish (HAZ) and low aggression zebrafish (LAZ). a Principal component analysis plot of the top 200 most variable genes after differential expression analysis between F4 HAZ and LAZ without mirror exposure. n = 3/group. b Volcano plot of differentially expressed genes (DEGs; padj < 0.05 and LFC > |2|) between F4 HAZ and LAZ at baseline. DEGs with the lowest adjusted p values (padj) are highlighted. n = 3/group. c Heat map displaying DEGs between F4 HAZ and LAZ at baseline. Hierarchical clustering of samples and genes reveals large differences between HAZ and LAZ, but similar transcriptional patterns within the two lines. n = 3/group. d Principal component analysis plot of the top 200 most variable genes after differential expression analysis between F4 HAZ and LAZ displaying similar aggression levels after mirror exposure. n = 6/group. e Heatmap of DEGs between female HAZ (HAZf) and female LAZ (LAZf) after mirror exposure. Hierarchical clustering of samples and genes reveals large differences between HAZ and LAZ, but similar transcriptional patterns within aggression subgroups. n = 3-4/group. f Heatmap of DEGs between male HAZ (HAZm) and male LAZ (LAZm) after mirror exposure. Hierarchical clustering of samples and genes reveals similar differences like in corresponding male animals. n=2–3/group. g Volcano plot displaying DEGs between HAZf and LAZf after mirror exposure. DEGs with lowest adjusted p values (padj) are highlighted. n = 3–4/group. h Volcano plot displaying DEGs between HAZm and LAZm after mirror exposure. DEGs with the lowest adjusted p values (padj) are highlighted. n = 2–3/group. Golden dots in Volcano plots indicate genes upregulated in HAZ more than log fold change 2, blue dots represent genes downregulated in HAZ more than LFC − 2 and black dots represent genes not passing these thresholds. Source data and individual data values are available at the ebrains data repository, DOI: 10.25493/VTP5-8J9 and in Additional file 2