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Figure 3—figure supplement 1.

ID: ZDB-IMAGE-220507-26
Source: Figures for Thumberger et al., 2022

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Figure Caption

Figure 3—figure supplement 1. Representative indel spectrum for each <italic toggle='yes'>Cas9</italic> mRNA used in the cell culture assay.

Indel spectrum diagram obtained from Tracking of Indels by Decomposition (TIDE) (red bargraphs) and Inference of CRISPR Editing (ICE) (blue bargraphs) analyses following JDS246-Cas9 mRNA, GeneArt CRISPR nuclease mRNA, and heiCas9 mRNA and Prx tracrRNA/crRNA transfections. Note decreased number of wild-type alleles (gray dashed line in TIDE analysis) in heiCas9-transfected cells and increased abundance of 26 nt deletion (black arrowhead in ICE analysis).

Acknowledgments

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