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Figure 3
Figure 3. Real-time quantitative PCR (RT qPCR) of atrogin-1 mRNA expression level in the whole body of 120 hpf zebrafish larvae exposed to lovastatin (LOV) and L-carnitine (LC) treatment. Bar graph demonstrates atrogin-1 mRNA expression level of zebrafish larvae in control (non-treated, NT; vehicle control, VC; 100 μM L-carnitine, LC100; 200 μM L-carnitine, LC200) and experimental (0.5 μM lovastatin, LOV; 0.5 μM lovastatin and 100 μM L-carnitine, LOV+LC100; 0.5 μM lovastatin and 200 μM L-carnitine LOV+LC200) groups. Expression of atrogin-1 mRNA was normalised to rpl13a (ribosomal protein L13a). A.U., arbitrary unit. Error bars show the standard deviation. The tables below indicate the pairwise comparison between atrogin-1 expression levels in investigated groups. Statistically significant differences are indicated with *; * p < 0.05 (Student’s t-test). The experiment was performed 3 times (with 25–30 individuals in each investigated group).
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