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Figure 7—figure supplement 3. Amplicon-seq of <italic toggle='yes'>ube2b-R8</italic> evoBE4max editants.

(a) Illumina sequencing of the target region in a subset (n = 3) of gDNA samples (single embryos) reveals quantitative target C5-to-T editing efficiencies as well as indel frequencies by. Aligned Illumina-reads analyzed, 21,768 (control); 24,613 (evoBE4max rep1); 57,332 (evoBE4max rep2); 51,273 (evoBE4max rep3). (b) Sequence composition following Amplicon-seq analysis surrounding the ube2b-R8 sgRNA target site ± 5 bp.

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