ZFIN Image: Cornean et al., 2022, Figure 7

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Figure Caption

Figure 7—figure supplement 2. Cytosine base editing allows the introduction of human CVD-associated missense mutations in medaka in F0.

(a–d) Following microinjection at the 1 cell stage of evoBE4max together with the respective sgRNAs, Sanger sequencing results were quantified by EditR (Kluesner et al., 2018). Results are plotted for the genomic loci of dapk3-P204 (n = 7), ube2b-R8 (n = 5), usp44-E68 (n = 11), and ptpn11-G504 (n = 11) and are depicted for the underlying phenotype (square: normal; circle: cardiac) according to the phenotypes grouped in supplement 3. The standard base editing window is depicted in blue. To highlight the dinucleotide context, the nucleotide preceding the target C is shown by red (A), green (T), blue (C) and yellow (G) squares below the respective C. (a’-d’) Sanger sequencing reads within the region of edited amino acids for the respective sgRNA experiment and locus. (b’-d’) sgRNAs mediating the C-to-T transition map to the complementary strand. Note: nucleotide position 27 shows a G/A SNP in the Cab genetic background used in this study for ube2b.

Acknowledgments

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