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Figure 1—figure supplement 3.

ID: ZDB-IMAGE-220426-40
Source: Figures for Cornean et al., 2022

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Figure 1—figure supplement 3. Details on selected sgRNA in ACEofBASEs.

Overview of sgRNA oca2-Q333 with target sequence, potential codon changes, and oligonucleotides required for cloning into the DR274 in vitro transcription vector for use with cytosine (a) and adenine base editors (b). All sgRNAs designed and tested were cloned with 5' substitution. A list of genomic targets is shown below, including a list of potential DNA off-target sequences (OTs) provided with sequence coordinates, mismatches (shown in red), gene name, and gene ID.

Acknowledgments

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