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Figure 1—figure supplement 1.

ID: ZDB-IMAGE-220426-37
Source: Figures for Cornean et al., 2022

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Figure Caption

Figure 1—figure supplement 1. Basic mechanism of cytosine base editing exemplifying the base editing principle.

(a) The cytosine base editor (CBE) complex will be guided to the genomic locus of interest by the selected sgRNA. While the protospacer adjacent motif (PAM) is essential for target recognition the editing window on the protospacer is, however, more volatile depending on the editor used and resides between positions 4 and 8 for standard CBEs and ABEs. (b–d) Mechanism of CBE-DNA binding, DNA opening and hydrolytic deamination. Following cellular DNA repair or replication processes target cytosines are converted to thymines. Note that within the hypothetical editing window any cytosine may be deaminated.

Acknowledgments

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