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Figure Caption
Fig. 2.
pthlha is expressed in dorsal and ventral subdomains of ch cartilage progenitors prior to differentiation. (A,B) In situ hybridization for pthlha mRNA at 66 hpf (A) and 72 hpf (B). The ch cartilage is outlined. White arrowheads indicate pthlha expression zones. (C-H) pthlha HCR in sox10:lyn-EGFP;entpd5a:KillerRed double-transgenic embryos at 48 hpf. F-H show magnified views of the outlined ch cartilage. entpd5a:KillerRed is not detected at 48 hpf. Ventral views, anterior to the left. ch, ceratohyal; D, dorsal; V, ventral. Scale bars: 100 μm (A,B); 50 μm (C-H).
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