ZFIN Image: Meng et al., 2021, Fig. 5

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Figure Caption

Fig. 5 Comparison of gene expression in cardiomyocytes induced by WNT inhibitor, P38 inhibitor, and nicotinamide.

A Hierarchical clustering analyses of differentiated cells with the indicated treatments. IWP-2 at 3 μM was added from day 2 to day5, and nicotinamide (Nam) at 20 mM or P38 inhibitor SB202190 (P38i) 10 μM were added from day 1 to day 5. The samples were harvested for microarray after 10 days of differentiation. B Enrichr analysis results showing the top 10 cell types enriched by nicotinamide, P38 inhibitor, or IWP-2 based on gene expression profiles. Venn diagram showing the numbers of genes upregulated (C) and downregulated (D) by nicotinamide (Nam), SB202190, or IWP-2 compared with untreated control. E The top 10 pathways enriched by the 446 genes upregulated by nicotinamide (Nam) and P38 inhibitor (P38i), but not IWP-2. F The top 10 pathways enriched by the 801 genes downregulated by nicotinamide (Nam), or P38 inhibitor (P38i), but not IWP-2. Comparison of gene expression related to heart development (G) and cell cycle (H) in the differentiated cells generated under different treatments after 10 days of differentiation.

Acknowledgments

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