ZFIN Image: Hsu et al., 2022, Figure 7

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Figure Caption

Figure 7

RNA-Seq reveals the direction of rorα possible downstream genes in neutrophil migration. (A) Volcano blot of DEGs with significant expression changes in rorα DN neutrophils. Cyan: down-regulated differentially expressed genes (DEGs); Magenta: up-regulated DEGs. (B) Significant gene function enrichment of human homologs of genes in the down-regulated DEGs. The heat map indicates the log2 fold change of the selected DEGs. The relevance of individual DEGs in each pathway is indicated with purple. (C) Venn diagram showing human homologs of genes in the down-regulated DEGs overlapping with putative RORα target genes in THP-1 or HUVEC cells. (D) Transcript level validation of downregulated genes in neutrophils sorted from rorα DN line zebrafish. Results from three independent experiments are normalized to rpl32 and presented as mean ± s.d., students’ t-test.

Acknowledgments

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