ZFIN Image: Korte et al., 2022, Figure 5

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Figure Caption

Figure 5

Transformed keratinocytes exhibit increased proliferation and reduced apoptosis during tumor initiation in thioredoxin mutants. (A) EdU staining (white) of txn^+/+ or txn^-/- larvae with KRas^G12V-expressing basal keratinocytes (magenta) at 3dpf. (B) Quantification of the frequency of EdU-positive KRas^G12V-expressing basal cells 3dpf in txn wt (n=35) and mutant (n=35) larvae. (C) Cleaved caspase-3 immunofluorescence of txn^+/+ or txn^-/- larvae. (D) Quantification of relative signal intensity within transformed cells 3dpf in txn^+/+ (n=45) and txn^-/- (n=51) larvae. Scale bar, 10um. Large, bolded shapes indicate average value per replicate (n), with small shapes representing data points from independent larvae. Samples were analyzed for statistical significance via t-test (p < 0.001***, p < 0.0001****).

Acknowledgments

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