Fig. 5.

Fig. 5. Mobility patterns of YFP-H-Ras and the constitutively active mutant YFP-H-Ras^V12, in epidermal cells of the zebrafish embryos after treatment with Latrunculin B (LatB) and methyl-β-cyclodextrin (MBCD). (A-C) Plots representing mobility patterns of the wild-type YFP-H-Ras. (A) Fraction size of the fast-diffusing population (α), plotted against the time lag. (B) Mean squared displacements plotted against the time lag for the fast-diffusing fraction ((r²₁). (C) Mean squared displacements plotted against the time lag for the slow-diffusing fraction (r²₁). (F) Mean squared displacements plotted against the time lag for the slow-diffusing fraction (r²₂). Parameters obtained upon curve fitting are presented in Table 1. To establish the values of dynamic parameters, three different embryos per each treatment group (vehicle, LatB and MBCD) and construct (YFP-H-Ras^V12, YFP-H-Ras) were imaged on each of the three different experimental days. Each data point is presented in the form of a mean±s.e.m., and the 95% c.i. of the mathematical fit is shown. Shapiro–Wilk statistical test was performed to check for normality of the data set. Statistical analysis was performed using a one-way ANOVA [for both H-Ras^WT and H-Ras^V12P(r²₂)>0.05 and P(α r²₁)<0.05 at a t_lag of 25 ms] with a Tukey's range post-hoc test (for details of the post-hoc test results, see Table 2).