ZFIN Image: DiNapoli et al., 2022, FIGURE 5

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Figure Caption

FIGURE 5

H3.3K27M accelerates melanoma formation. (A) Kaplan-Meier survival curves of Tg(mitfa:BRAF(V600E)); tp53^zdf1/zdf1; mitfa^w2/w2 zebrafish injected with indicated miniCoopR constructs. p value shown calculated from log-rank test between miniCoopR H3.3 and H3.3K27M. (B) Representative tumor-bearing miniCoopR H3.3K27M animal. (C) Western blot of indicated histone marks from miniCoopR H3.3 and H3.3K27M tumors. (D) Top differentially expressed genes between miniCoopR H3.3 and H3.3K27M. Genes with differential expression p < 1E05 are shown. (E) Gene set enrichment analysis (GSEA) of miniCoopR H3.3 and H3.3K27M tumors. NES, normalized enrichment score; FDR, false discovery rate; F, normalized read count of ezh2 from miniCoopR H3.3 and H3.3K27M tumors. p = 6.37E-06, log₂ fold change = −1.52. (G) overall survival of human melanoma patients (N = 180) stratified into low (blue) or high (red) expression of 50 gene signature developed from miniCoopR H3.3K27M tumors.

Acknowledgments

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