ZFIN Image: Cook et al., 2021, Fig. 2

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Figure Caption

Fig. 2 Small-molecule inhibition of other putative kinase effectors fails to promote alkaline phosphatase expression. A, for all other kinases that displayed >70% inhibition by DIPQUO in the profiling assay, C2C12 cells were treated for 3 days with commercially available small-molecule inhibitors in the concentrations shown and stained for ALP expression. Inhibitors were as follows: dorsomorphin (DM), PV1019 (PV), Chk2inhII (Chki), RO3306 (RO), roscovitine (Rosco), CRT006 (CRT), XMU-MP-1 (XMU), Bosutinib (Bosu), LRRK2-IN-1 (LRRKi), and GSK2578215a (GSK257). The scale bar represents 200 μm. B, cells were similarly treated with dual combinations of the same kinase inhibitors (for each kinase, only one inhibitor was chosen based on availability) to assess whether simultaneous inhibition of different pathways would synergize to result in ALP expression. Treatments were performed at the same concentrations, with the exception of CHIR treatment, which was used at a concentration determined empirically to be subthreshold for ALP staining. For all panels, representative images are shown from experiments that were performed in at least three biological replicates. The scale bar represents 200 μm. ALP, alkaline phosphatase; CHIR, Wnt signaling activator CHIR99021.

Acknowledgments

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