ZFIN Image: Codolo et al., 2022, Figure 5

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Figure 5

HP-NAP administration in zebrafish promotes the acquisition of a pro-inflammatory profile by macrophages. Tg(mpeg1:EGFP)gl22 zebrafish embryos were xenotransplanted with M121224 melanoma cells and treated with HP-NAP or saline (vehicle) at 2 dpi. After 24 h (3 dpi) macrophages were FACS-sorted and the expression of genes was evaluated by qRT-PCR. Data were normalized to the housekeeping gene 18S. The expression of each gene in macrophages isolated from HP-NAP-treated fishes was relative to that in macrophages isolated from vehicle animals, taken as reference and set as 1. Data are expressed as the mean ± SEM of 2 independent experiments and analyzed by Student’s t test; *, p < 0.05; ***, p < 0.001.

Acknowledgments

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