Figure 5

Regular movements observed in the distal intestine at 4 and 7 dpf and peristaltic reflex-like activity observed at 7 dpf without feeding. Kymgraphs of the bright field and GCaMP3 fluorescence movies, at 4 dpf (a) and 7 dpf (b). Regular movements (white triangles) were not always associated with Ca²⁺ events in the circular smooth muscles, although weak Ca²⁺ events could be sometimes associated with the regular movements, as indicated by asterisks in (a). On the other hand, peristaltic reflex-like movement (magenta triangles) which was sometimes observed without feeding was associated with strong Ca²⁺ events in the circular smooth muscles.