ZFIN Image: Ziegler et al., 2021, Fig. 3

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Figure Caption

Fig. 3

Protein translocation in living zebrafish embryos.

a, Schematic illustration of workflow for in vivo application in zebrafish embryos. Fertilized eggs were injected with vectors for LYN–mCherry–PYR^Mandi or TOM20–mCherry–PYR^Mandi and eGFP–ABI expression, resulting in mosaic expression of target proteins at 3–5 dpf. b–d, Confocal microscopy images of different cell types (fin cells (b), epithelial cells (c) and muscle cells (d)) in living zebrafish embryos expressing receiver and plasma membrane-localized receptor domains before and 10–20 min after addition of 500 nM Mandi are shown. Data are representative of ≥three independent experiments for each cell type; scale bar, 40 µm.

Acknowledgments

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