Fig. 3

Nrp2a and nrp2b likely work in parallel and targeting errors persist for days. A. Representative confocal sections of 3 dpf wild type and nrp2a;nrp2b double mutant siblings showing multiple misprojections by TRPC2-class OSNs in the MG, DZ, and LG3 protoglomeruli. Yellow arrows indicate misprojecting axons. B. TRPC2-class OSNs in nrp2a;nrp2b double mutants project axons to more ectopic termination sites than single mutant or wild type siblings. C. Representative confocal sections of 6 dpf wild type and nrp2a;nrp2b double mutant siblings showing multiple misprojections by TRPC2-class OSNs in the MG and LG3 protoglomeruli. Yellow arrows indicate misprojecting axons. D Targeting errors of TRPC2-class OSNs persist into later stages of development. The number of ectopic termination sites per olfactory pit is significantly higher in nrp2a;nrp2b double mutant as compared to wild type siblings at both 3 dpf and at 6 dpf