ZFIN Image: Bertho et al., 2021, Fig. 2

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Figure Caption

Fig. 2 (A) RT-PCR of indicated genes. Expression from the ziwi promoter is detectable by d10 and buc is detectable later by d31. gRNAs are expressed in transgenic animals identified by GFP+ hearts. cas9 is expressed as expected for the respective promoters. (B) Western blot to detect maternal Cas9 protein (asterisk) in transgenic eggs from GMS:buc transgenic female (2 hpf; 10 egg equivalents/well). (C,D) Expression of GFP in ovary (C) and testis (D) of adult GMS transgenic animals. (E,F) Expression of GFP in ovary (E) but not testis (F) of adult OMS transgenic fish. Four ovaries and four testes were dissected and examined for each transgene. The testis is outlined

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