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Figure 2
Effect of Cas9/sgRNA concentration on lethality, cutting efficiency, and phenotype outcome in zebrafish. Different concentrations of Cas9 protein mixed with 4× sgRNA guides against the gene tyrosinase (tyr) were injected into the yolk of one-cell stage zebrafish embryos. Injected animals were then observed at 2 dpf to score toxicity (malformation or death), partial depigmentation, or total depigmentation (suggesting complete bi-allelic tyr-knockout). A mix of 400 pg of Cas9/sgRNAs was selected to be used routinely in our pipeline.
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