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FIGURE 4
ALG-AgNPs significantly inhibit the growth of cytosolic Mycobacterium tuberculosis in a macrophage infection model. (A) Schematic diagram of the experimental procedures. (B–E) THP-1 cells were differentiated into macrophages by PMA stimulation for 3 days, then infected with different strains of Mtb at a MOI of 1 for 24 h. After treatment with PBS, or different amounts of ALG-AgNPs (25, 50, and 100 μg/ml), or rifampicin (RIF) (1 μg/ml) for 5 days, the cells were lysed and plated on 7H10 agar plates. The bacterial growth was determined by CFU of (B) H37Rv, (C) W6 (Beijing strain), (D) KVGH264 (MDR), and (E) TCHL017 (XDR). MDR: Multidrug-resistant TB; XDR: extensively drug-resistant TB. Data represent mean ± SEM of 3 experiments. ***p < 0.001.