ZFIN Image: Tsedeke et al., 2021, Fig. 3

Image

Figure Caption

Fig. 3 The tnni4b.3 reporter is expressed by cardiomyocytes starting at early larval stages in zebrafish. (A-C″) Confocal images (maximum intensity projections) of wholemount Tg(tnni4b.3:NLS-EGFP); Tg(myl7:BFP-CAAX) hearts at 72 (A-A″), 96 (B–B″) and 144 (C–C″) hpf. White arrowheads point to EGFP+ CMs at 96 hpf (B–B″). White dashed lines outline the hearts. (D-E″) Mid-sagittal (D, E) and wholemount 3D rendered (D′, D″, E′, E″) confocal images of Tg(tnnc2:NLS-tdTomato); Tg(tnni4b.3:NLS-EGFP); Tg(myl7:BFP-CAAX) hearts at 72 (D-D″) and 96 (E-E″) hpf. tnnc2:NLS-tdTomato+ CMs located in the trabecular layer are highlighted in blue. Magenta arrowhead points to an NLS-tdTomato+/NLS-EGFP+ outer curvature CM. V: ventricle; At: atrium. Scale bars: 20 μm.

Acknowledgments

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Reprinted from Developmental Biology, 476, Tsedeke, A.T., Allanki, S., Gentile, A., Jimenez-Amilburu, V., Rasouli, S.J., Guenther, S., Lai, S.L., Stainier, D.Y.R., Marín-Juez, R., Cardiomyocyte heterogeneity during zebrafish development and regeneration, 259-271, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.