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Figure 5 Comparison of heart rate regularity and cardiac rhythm measurement in IBMX treated zebrafish embryos aged at 72 hpf using either OpenCV (red color) or ImageJ (blue color) methods. Comparison of atrium to atrium (A-A) (A) and ventricle to ventricle (V-V) (B) intervals using either ImageJ or OpenCV methods. Comparison of atrium-ventricle (A-V) (C) and ventricle to atrium (V-A) (D) intervals using either ImageJ or OpenCV methods. Comparison of heartbeat regularity in either atrium (E) or ventricle (F) using either ImageJ or OpenCV methods, red dotted line signified the normal level of each parameter obtained from control group. Data was calculated using t-test (A,B) and Wilcoxon test (C–F), and presented as Mean ± SD, n = 10 (* p < 0.05; ** p < 0.01).