Fig. 2.

Fig. 2.

+/+, +/− , and –/– refer to sibling-controlled wild-type and mutant fish. (A) Left: At 7 dpf, scn1lab^+/− fish are more dispersed than wild-type siblings (P_Fisher ≈ 0.036, N_+/+ = 10, N_+/− = 16 groups, Cohen’s d = −0.8). Dashed lines represent values of shuffled groups. At 21 dpf, fish are more aggregated. Scn1lab^+/− aggregate less than scn1lab^+/+ (P_Fisher ≈ 0.0001; N_+/+ = 25, N_+/− = 26 groups, Cohen’s d = −1.09). Right: Group alignment increases with age; however, we could not detect an effect of the scn1lab mutation (P_{7 Fisher} = 0.26, P_{21 Fisher} = 0.47). *P < 0.05, ***P < 0.0005. (B) Left: At 7 dpf, disc1^−/− are less dispersed than wild-type siblings (P_Fisher ≈ 0.0128; N_+/+ = 7, N_−/− = 17 groups, Cohen’s d = 1.04). At 21 dpf, disc1^−/− show more aggregation compared to wild-type siblings (P_Fisher ≈ 0.0129; N_+/+ = 12, N_−/− = 13 groups, Cohen’s d = 0.95). Right: We could not detect effect of the disc1 mutation on alignment (P_{7 Fisher} = 0.33, P_{21 Fisher} = 0.40). (C) Pearson’s correlation of alignment and aggregation in scn1lab_allele2 7-dpf (top) and 21-dpf (bottom) fish. Positive correlation for 21-dpf scn1lab_allele2^+/− fish. Uncertainty regions are the pointwise 95% CI of the linear regression model. (D) We could not detect correlation for disc1. (E) Left: scn1lab_allele2^+/− turn away more from visual clutter at 7 dpf (top) and turn toward clutter less at 21 dpf (bottom). Right: Integral of the curves symmetrized. Repulsion is negative. Attraction is positive. (F) Same as in (E) but for disc1. Mutants show a flattening of the 7-dpf repulsion curve (top) and an enhancement in 21-dpf attraction (bottom). Bold lines in (E) and (F) represent turning probability calculated from left/right turns of all fish in 5° bins; uncertainty regions are the pointwise 95% CI of a fitted binomial distribution to the events in each bin.