FIGURE 2

FIGURE 2

Figure 2. stub1 mRNAs are expressed in different tissues and in the Purkinje cells of adult zebrafish cerebellum. (A) Scatter plot of ddPCR data presenting the absolute number of stub1 transcripts per ng of cDNA for different zebrafish tissues. Each dot represents a biological replicate. Means and standard deviations are indicated by the lines and bars in the graph (n = 4). (B) DIG-labeled stub1 cDNA probes were synthesized, and used for visualization of stub1 mRNA expression in adult zebrafish brain sections, using in situ hybridization assay. (C) Detailed view of cerebellum where stub1 positive cells in the Purkinje cell layer are indicated by arrowheads. (D) Negative control corresponding to the same area as shown in (B). Tel, telencephalon; TeO, tectum opticum; TL, torus longitudinalis; Val, valvula cerebelli; Cce, Corpus cerebelli; EG, eminentia granularis; Mo, medulla oblongata; Hyp, hypothalamus; A, anterior; P, posterior; D, dorsal; V, ventral; ML, molecular layer; PCL, Purkinje cell layer; GL, granular layer.