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Figure 8
Figure 8. Induction of tumor metastasis in twist1a+/xmrk+ transgenic zebrafish via Dox and 4-OHT treatment under exposure to LPS or DSS. Twist1a+/xmrk+ transgenic zebrafish were treated with 60 μg/mL Dox and 1 μg/mL 4-OHT starting from 4 mpf with sampling performed at 4 wpi. (A,B) Immunofluorescence analysis of twist1a+/xmrk+ liver tumor metastasis at 4 wpi with corresponding H&E histological examination of liver sections. Scale bar: 50 or 200 μm. (C,D) Kaplan–Meier survival curves showing days post-induction plotted against percentage survival to 4 wpi. (E,F) Histological examination confirmed that at 4 wpi, all twist1a+/xmrk+ transgenic zebrafish developed HCC or metastatic HCC. Differences among the variables were assessed using one-way ANOVA. Statistical significance: *** p < 0.001.
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