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Figure 5
Figure 5. Assessment of E-cadherin and vimentin staining in instances of HCC progression in twist1a+/xmrk+ double transgenic zebrafish. Immunohistochemical staining was performed on liver paraffin sections from WT, twist1a+, xmrk+, and twist1a+/xmrk+ zebrafish at 4 wpi. Staining of markers of EMT activation: (A) E-cadherin and (B) vimentin. Scale bar: 50 μm. Quantification of the percentage of cells testing positive for (C) E-cadherin and (D) vimentin. Differences among the variables were assessed using Student’s t-tests. Statistical significance: ** p < 0.01, *** p < 0.001.
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