ZFIN Image: Peron et al., 2021, Fig. 3.

Image

Figure Caption

Fig. 3.

Mutation of Stat3 DBD (DNA binding domain) does not affect its mitochondrial activities. (A) WISH with anti-mt_nd2 antisense probe in uninjected 48 hpf larvae and in 48 hpf larvae injected with MLS_mStat3_NES or MLS_mStat3_ΔDNAbd_NES mRNA. (B) qRT-PCR showing mt_nd2 gene expression after injection of MLS_mStat3_NES or MLS_mStat3_ΔDNAbd_NES mRNA in 48 hpf embryos; zgapdh was used as internal control. (C) IF on ESCs transiently transfected with MLS_mStat3_NES or MLS_mStat3_ΔDNAbd_NES and stained with anti-STAT3 (green), anti-ATAD3 (red) antibodies and DAPI (blue). Data are mean±s.e.m. *P<0.05; **P<0.01 (unpaired two-tailed t-test on three independent biological samples, where n not specified). Scale bars: 200 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development