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Figure 4
Effect of TRIM28 nanobody NB237 on invasion of GB cells. (A,B) GB cells, U373 and U87, at 10,000 cells/insert; and (C) GSCs NCH421k at 80,000 cells/insert, were seeded in the upper compartment in serum-free medium alone or in combination with TRIM28 nanobody (final concentration 100 µg/mL). The cells that invaded the Matrigel after 48 h were stained with 0.1% crystal violet and counted using inverted microscope. Each value represents mean ± S.D. (Three biological replicates). Statistical analyses was performed using GraphPad Prism software, using t-test, * p < 0.05, vs. control group.
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