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Figure 4
Melanophore dish assay comparing α-MSH variants from different species. (a) Schematic of melanophore dish assay with wild-type (WT) larvae. Red asterisks indicate imaging timepoints. (b) Results of melanophore dish assay comparing mean melanophore area (n = 30–50 melanophores from 3–5 larvae) calculated after induction of melanosome dispersion by treatment of the respective α-MSH peptide. One-way ANOVA with post-hoc Dunn’s multiple comparison test was used for statistical significance between groups; data represented as mean ± SEM; *** p < 0.001, ns = not significant. (c) Normalization of mean melanophore area by initial area of the control group (see Equation (1)).