ZFIN Image: Pinto et al., 2021, Figure 4

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Figure Caption

Figure 4

The zebrafish OP has two different types of cilia. (A) Schematic representation of 5 dpf zebrafish head, structures of interest are marked with arrows—olfactory pits (OP). (B) TEM low magnification image showing a cross-section across an OP of a WT zebrafish, a bowl-shaped structure containing multiciliated cells and cilia in several orientations. In the periphery of this pit (dotted boxes a and inset), cilia containing classical motile structure 9 + 2 with dynein arms (black arrowheads). In the most internal region of the OP (dotted box b and inset) we detected cilia with 9 + 2 ultrastructural arrangement without dynein arms (red arrowheads); this pattern was visible in WT embryos (n = 3). (C) Snapshot from a movie of beating OP cilia and respective heatmap by software CiliarMove (C’), highlighting a region of immotile cilia (arrowhead) that was coincident with the region detected by TEM in B. n—nucleus; cb—cilia border; OP—olfactory pit. Thin bar 6 µm, thick bar 100 nm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.