ZFIN Image: Islam et al., 2021, FIGURE 9

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Figure Caption

FIGURE 9

DNA double strand break and recombination marker Rad51 was expressed in smc1b^Q198X mutant spermatocytes. Immunolabeling of Rad51 (green) and Sycp1 (magenta) in wild-type and mutant spreads, combined with telomere (telo) in situ hybridization (orange). (A,B) Rad51 protein was detected in wild-type beginning in leptotene stage and was initially located near the chromosome ends in leptotene and bouquet stages. (C,D) In more advanced wild-type spermatocytes, Rad51 localization become more distinct and resolved to one or two spots per chromosome. (E,F) Mutant spermatocytes had detectable levels of Rad51. (G) Quantification of Rad51 signal in wild-type (N = 10) and mutant (N = 15) leptotene stage cells demonstrates there is no significant difference (P = 0.0614). The horizontal bars show the medians. Scale bar is 10 μm.

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Acknowledgments

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