ZFIN Image: Luo et al., 2021, Fig. 1

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Fig. 1

Generation of zebrafish with tissue-specific pten or tp53 deficiency. a The driver construct provides zebrafish fabp10 promoter-driven expression of Cre, which can bind to the loxp-stop-loxp cassette in the responder construct and activate the expression of Cas9-P2A-mCherry and the gRNA via the fabp10 and U6 promoters, respectively. The expression of Cas9 can be determined by mCherry fluorescence in zebrafish larvae. The driver and responder constructs are flanked by Tol2 transposon sites. b Strategy for the generation of transgenic zebrafish lines. The driver and responder constructs were mixed and injected with Tol2 mRNA to generate mosaic founder transgenic fish lines. c The phenotype of the F₂ transgenic fabp10^WT larvae at 3 dpf. Scale bars, 1 mm. d Colocalization of mCherry (red) and endogenous Fabp10 (green, FITC-labelled) expression in liver tissues of fabp10^WT larvae at 3 dpf. Scale bars, 400 μm

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Clin. Cancer Res.