Figure 6
            
        
    
    
        
        
        
             (A) Immunofluorescence with atrium-specific S46 antibody allows distinction of the cardiac chambers. (B) Quantification of ventricular and atrial cardiomyocytes in wt and oug mutant embryos at 2dpf. (C) Explanting oug mutant hearts and culturing them in vitro, ex-embryo does not rescue defective looping. (B): Horizontal bars: mean value ± SEM. Legends: R: Right; L: Left; S: Superior side; I: Inferior side . Scale bars: 100 µm. 
        
    
Defective looping in oug mutants is not due to reduced cardiomyocyte number or embryonic environment.
 
                
                    
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                        Acknowledgments
                    
                    
                
                
            
        
        
    
        
            
            
                
                    
                        
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