ZFIN Image: Topel et al., 2020, Fig. 4

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Figure Caption

Fig. 4

HOTAIR OE induced c-Met inhibition decreases adhesion, proliferation and colony formation. Normalized cell index and slope analysis of adhesion to (a) cell culture treated and (b) basal membrane extract-coated surfaces analyzed with xCELLigence RTCA. (c) RT-qPCR analysis of ITGA6, ITGB1, ITGA4 and ITGB4 expressions. (d) Analysis of normalized cell index and rate (slope) of proliferation by xCELLigence RTCA system. Normalized (e) MTT absorbance (570 nm) and proliferation rate (slope) calculated by MTT absorbance. 2D colony formation analysis of MOCK and HOTAIR clones presented as (f) percentage of colonization, (g) percentage of area covered by colonies, (h) intensity of Giemsa-staining (i) average colony size in pixels and (j) representative images of clones generated with ImageJ plugin ColonyArea. 2D colony formation is analyzed by ImageJ plugin ColonyArea. All graphs of experiments are presented as the mean ± SEM of at least 3 independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

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