IMAGE

Fig. 7.

ID
ZDB-IMAGE-210801-93
Antibodies
Source
Figures for Santra et al., 2021
Image
Figure Caption

Fig. 7.

Reduced mitochondrial membrane potential contributes to hair cell death in V-ATPase mutant neuromasts. (A) The vital dyes MitoTracker and TMRE were used to assess mitochondrial mass and mitochondrial transmembrane potential, respectively, in live wild-type and atp6v1f−/− embryos at 4 dpf. Dashed line circles indicate the neuromast boundary. (B,C) Quantification of mean fluorescence intensity measurements per neuromast of MitoTracker (B) and TMRE (C) in wild-type and atp6v1f−/− embryos at 4 dpf. (D) Ratio of TMRE fluorescence intensity to MitoTracker. n=number of embryos. ****P<0.0001 by unpaired Student's t-test with Welch's correction. (E) Representative images of acetylated tubulin immunostaining of hair cells in wild-type and atp6v1f−/− embryos at 4 dpf after treatment with DMSO (vehicle control), CsA or RU360 from 2 dpf to 4 dpf. Arrowheads point to individual hair cells. (F,G) The number of hair cells per neuromast in control embryos and embryos treated with CsA (F) or RU360 (G). n=number of embryos. *P<0.04 by two-way ANOVA with Bonnferroni–Šidák post hoc test.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.