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Figure 2—figure supplement 1.

ID: ZDB-IMAGE-210716-63
Source: Figures for Zilova et al., 2021

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Figure Caption

Figure 2—figure supplement 1. Medaka primary embryonic pluripotent cells acquire retinal fate cell autonomously, independently of cell aggregation.

(a) Schematic representation of primary embryonic pluripotent cell culture. Blastula-stage embryos were dissociated and cultured either under rocking suspension culture condition or as individual cells in wells of 96-well plates. (b) Overlay of bright-field and fluorescence images of cells derived from Rx3::H2B-GFP transgenic fish and cultured for 24 hr under rocking suspension culture conditions. Magnification shows three individual clusters of cells. Dashed line outlines the borders of an individual cluster. (c) Overlay of bright-field and fluorescence images of cells derived from Rx3::H2B-GFP transgenic fish cultured individually in wells of 96-well plates at time 0.5 hr (just about 30 min after dissociation and seeding) and 19 hr of the culture. Scale bars: 100 μm.

Acknowledgments

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