FIGURE 6

FIGURE 6

Neuromuscular junctions on jaw muscles in olt mutants are unaffected. (A–D) Ventral view with anterior to the left of the jaw musculature in 7 dpf Tg(isl1:GFP);Tg(α-actin:GFP) larvae. (A,B) SV2a antibody staining (magenta) of presynaptic structures on the jaw muscles (green). (C,D) Postsynaptic Acetylcholine receptor (AChR) clusters were labeled with alpha-bungarotoxin (αBTX) (magenta) on the jaw muscles (green) in live larvae. Volumes of the presynaptic structures and postsynaptic clusters were calculated from 3D renderings by using the muscle as a mask. There was no significant difference in the volumes of SV2a-stained structures (E) or αBTX-labeled structures (F) on any muscle groups between wildtype siblings and olt mutants. Data pooled from 3 experiments (number of larvae in parenthesis). The two-tailed student t-test was performed to test for significance. NS, not significant. ima/imp, intermandibularis anterior/intermandibularis posterior; ih, interhyal; hh, hyohal; am, adductor mandibularis; sh, sternohyoideus. Scale bar (A,C), 100 μm.