ZFIN Image: Asante et al., 2021, FIGURE 6

Image

Figure Caption

FIGURE 6

Neuromuscular junctions on jaw muscles in olt mutants are unaffected. (A–D) Ventral view with anterior to the left of the jaw musculature in 7 dpf Tg(isl1:GFP);Tg(α-actin:GFP) larvae. (A,B) SV2a antibody staining (magenta) of presynaptic structures on the jaw muscles (green). (C,D) Postsynaptic Acetylcholine receptor (AChR) clusters were labeled with alpha-bungarotoxin (αBTX) (magenta) on the jaw muscles (green) in live larvae. Volumes of the presynaptic structures and postsynaptic clusters were calculated from 3D renderings by using the muscle as a mask. There was no significant difference in the volumes of SV2a-stained structures (E) or αBTX-labeled structures (F) on any muscle groups between wildtype siblings and olt mutants. Data pooled from 3 experiments (number of larvae in parenthesis). The two-tailed student t-test was performed to test for significance. NS, not significant. ima/imp, intermandibularis anterior/intermandibularis posterior; ih, interhyal; hh, hyohal; am, adductor mandibularis; sh, sternohyoideus. Scale bar (A,C), 100 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neural Circuits