Figure 3

Figure 3

Characterization of binocular saccades. (a) Heat scatter plot⁴⁸ showing the distribution of binocular optokinetic saccade magnitudes in all analyzed calcium imaging experiments (n = 30 larvae, 146 recordings, 9,866 data points). The saccades shown in Fig. 2b are plotted in red. Only saccades larger than 4° were included in further analysis for technical reasons. The classification thresholds for monocular, clockwise (CW), counter-clockwise (CCW), convergent (CONV) and divergent (DIV) are indicated as dashed grey lines, and the outer quadrants are labelled in red. (b) Proportion of saccade types per protocol phase. Note that the translation and unbalanced monocular stimulus phases elicited higher proportions of disconjugate saccades than the rotation phase. (c) Dependence of maximum saccade velocity on saccade magnitude (main sequence). Spontaneous clockwise saccades of both eyes (LE, RE) were recorded at 750 fps (n = 24 zebrafish larvae). A histogram of saccadic velocities is shown in brown on top. The distribution was fitted using a cubic polynomial with a resulting R² value of 0.5. LETN left eye temporal nasal; RENT right eye nasal temporal; LENT left eye nasal temporal; RETN right eye temporal nasal; mono: monocular saccade.