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Figure 3 Caffeine-treated embryos display a reduced expression of fli1 and cad5. Representative lateral views of the WISH for fli1 (A) and cadh5 (B) probes, performed in 28 hpf embryos raised in sterile water (control) or treated with caffeine 100 mg/L. Magnification 32×. Ratios at the bottom right part of each picture specify the number of embryos showing the same staining pattern, compared to the total number of embryos used for each experiment (n = 15). (C,D) The bar diagrams show the quantification of the colorimetric signal in control and caffeine-treated embryos. The expression of fli1 and cadh5 was significantly reduced in the caffeine-treated embryos. For cadh5, two replicates were performed (n = 15), while for fli1, the experiments were conducted in triplicate (n = 15). * p < 0.05, ** p < 0.005, when compared with control group.