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Figure 6—figure supplement 1. Phylogenetic reconstruction of animal contractile cell transcriptional regulators.

Trees depict Csrp/Crip (A) and Fox group I (B) gene families. Genes labeled red are enriched in at least one contractile gene pair identified via SAMap. Support values indicate bootstrap support from 1000 nonparametric (Csrp) or ultrafast (Fox) bootstrap replicates. Besides these two transcriptional regulators, contractile cells in all seven species were found to be also enriched for transcription factors from the C2H2 Zinc Finger, Lim Homeobox, and Paired Homeobox families, though in different cell types we found enrichment of a number of distinct orthologs. Whether this reflects an ancestral role for these transcription factor families in regulating contractility or their independent evolution will require additional taxonomic sampling and broader coverage of muscle cell diversity to resolve.

Acknowledgments
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