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Figure 4 (A) UMAP projection of the combined manifolds. Tissue type annotations are adopted from the S. mediterranea atlas (Fincher et al., 2018). The schistosome atlas was collected from juvenile worms, which we found to contain neoblasts with an abundance comparable to that of planarian neoblasts (Li et al., 2021). (B) Overlapping expressions of selected tissue-specific TFs with expressing cell types circled. (C) UMAP projection of the aligned manifolds showing planarian and schistosome stem cells, with homologous subpopulations circled. Planarian neoblast data is from Zeng et al., 2018, and cNeoblasts correspond to the Nb2 population, which are pluripotent cells that can rescue neoblast-depleted planarians in transplantation experiments. (D) Distributions of conserved TF expressions in each neoblast subpopulation. Expression values are k-nearest-neighbor averaged and standardized, with negative values set to zero. Blue: planarian; yellow: schistosome.