Fig. 6

Fig. 6 (A-E″) Single confocal plane of representative dazl^Δ7/+ and dazl^Δ7/Δ7 at day 8, and dazl^Δ7/+, dazl^Δ7/Δ7 and dazlae^57/Δ7 gonads at day 10. Fish are transgenic for [ziwi:GFP], expressed zygotically in the germ cells. Gonads were immunostained with GFP (green, germ cells), Vasa (magenta) as a GC-specific cytoplasmic marker, and DAPI (gray; nuclei). (A-A″) GFP expression in Vasa+ GC of day 8 dazl^Δ7/+ (n=2, B-B″) and dazl^Δ7/Δ7 (n=2) gonads, and day 10 dazl^Δ7/+ (n=2, C-C″), dazl^Δ7/Δ7 (n=4, D-D″) and dazlae57/Δ7 (n=2, E-E″) gonads. Yellow dashed lines delineate the gonad.