ZFIN Image: Paasila et al., 2021, Fig. 1

Image

Figure Caption

Fig. 1 (a–c) Widefield epifluorescence images of (a) Syp‐immunostaining of presynaptic boutons, (b) Iba1‐immunostaining of a microglia profile, and (c) the composite image (case AD 1). Widefield images are shown as maximum intensity projections of 35 z‐slices with a depth of 6.65 µm; insets outline the fields of view corresponding to GSDIM reconstructions in the following row (d–f). (d–f) GSDIM reconstruction demonstrating the ability of the technique to display very high resolution images of (d) Syp‐immunostaining, (e) Iba1‐immunostaining, and (f) the composite image. The numbered spots in (f) highlight overlapping signal (white), that is, Syp‐positive presynaptic material inside Iba1‐positive microglial cell processes. Scale bar in c = 10 µm (a–c), in f = 5 µm (d–f)

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci. Res.