Fig. 8

Fig. 8

a Effect of PYO (10 nmol/g) on mice body weight in 6-week-old WT C57Bl6/J males (n = 4) and C57Bl6/J females (n = 4). Weekly measurements of mice body weight (grams) are shown. Data are means ± SEM. b Plasma proinflammatory cytokine levels (TNF-α, IL-1β, IL-6) from mice treated with PYO 10 nmol/g (n = 8 independent samples) were not significantly altered compared to untreated controls (n = 3 independent samples). Bars indicate mean ± SEM with superimposed individual data points. c Representative light micrographs of the liver, kidney, and brain sections from mice untreated or treated with PYO (10 nmol/g) for 2 months (n = 3 biological replicates). All organs were free from gross pathological changes on hematoxylin and eosin (H&E) staining. Scale bar: 100 μm. d Representative TEM images of the liver sections from mice untreated or treated with 10 nmol/g PYO. Nucleus, mitochondria, ER, and the other detected organelles did not show any visible alterations (n = 3 biological replicates). Scale bars are reported in each picture. e Representative oxyblot of the liver, heart, and brain lysates from mice untreated (U) or treated (1, 2, and 3) with PYO (10 nmol/g) for 2 months. Samples were either derivatized by adding DNPH (2, 4-dinitrophenyl hydrazine) solution (DER) to visualize carbonyl groups introduced by oxidative modifications, or treated with derivatization-control solution (CTRL). Vinculin was used as a loading control (means ± SEM, n = 3 independent samples). f Representative Western Blot and the relative quantifications of catalase expression levels observed in lysates of liver, heart, and brain samples lysates from mice untreated (U) or treated (1, 2, and 3) with PYO (10 nmol/g) for 2 months. Vinculin was used as a loading control (means ± SEM, n = 3 independent samples). For all panels, statistical significance was calculated using two-tailed Student’s t test.