ZFIN Image: Carver et al., 2021, Figure 1

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Figure Caption

Figure 1

Conserved structure of Adamts9 from C. elegans to human. Different function domains are illustrated in different color. Green triangles show sites for two commercial antibodies (Triple Point Biologics, Inc., OR) generated against 180 peptide sequence of prodomain and metalloproteinase domain of human Adamts9, which share 41% and 86% sequence identity with zebrafish Adamts9, respectively. Red triangle shows CRISPR targeting site for generating zebrafish adamts9 knockout. Two mutant zebrafish lines (∆10 and ∆11) which generated premature stop codons before the enzymatic active site (at 573aa) were selected and propagated²⁴ (Please see supplemental Figs. 1 and 2, Supplemental Tables 1–4 for detail).

Acknowledgments

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