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Figure Caption
Fig. 6 Photoconverted cardiomyocytes counted manually from a z-stack of the larval heart. A- 48 hpf left lateral side 3D stack of the heart, displaying every fourth stack of 2.0 μm. Red fluorescence shows right side lateral aLPM-derived Kaede-expressing cells photoconverted at 18 hpf. B-E Insets of the heart showing examples of how cardiomyocytes were counted with numbered points in yellow. This embryo had a total of 185 red fluorescent cardiomyocytes counted, which was verified by the average of three separate manually counted analyses.
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Reprinted from Developmental Biology, 472, Mao, L.M.F., Boyle Anderson, E.A.T., Ho, R.K., Anterior lateral plate mesoderm gives rise to multiple tissues and requires tbx5a function in left-right asymmetry, migration dynamics, and cell specification of late-addition cardiac cells, 52-66, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.