Fig. 5

Fig. 5 Asymmetric aLPM contributions to the larval heart are affected by Tbx5a knockdown. A- Schematic of experimental method with right side lateral aLPM photoconverted using 405 ​nm light (shown as blue) at 18 hpf. B- Bar plots of mean number of KaedePC cardiomyocytes at 48 hpf in each experimental condition. Points are counts from each embryo (Fig. S5). Statistics performed using the Wilcoxon Rank Sum test. C- Schematic at 48 hpf showing anterior (orange) versus posterior (blue) regions of the heart in dorsal and left lateral views. D-G- 48 hpf 3D projection of a confocal stack of the larval heart shows the contributions of red fluorescent KaedePC cells among green fluorescent Kaedenon−PC cells, D-E-WT embryos, anterior view. Dotted lines a-c are levels of individual Z-slices through the a-ventricle, b-atrium, and c-connecting region between the venous pole and pericardial sac. D- Heart derived from left side aLPM. E− Heart derived from right side aLPM. F-G- Tbx5a-deficient embryos, F- Heart derived from left side aLPM, anterior view. G- Heart derived from the right side aLPM, left lateral view. A-atrium; V-ventricle; asterisk-venous pole of the heart. Compass abbreviations: A-anterior; P-posterior; D-dorsal; V-ventral; R-right; L-left. Scale bar: 100 ​μm.