ZFIN Image: Acin-Perez et al., 2020, F

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Figure Caption

F <styled-content toggle='no' style='fixed-case'>RIFS</styled-content> measurement of Complex I, <styled-content toggle='no' style='fixed-case'>II</styled-content>, and <styled-content toggle='no' style='fixed-case' xmlns:xlink='http://www.w3.org/1999/xlink'>IV</styled-content> activity in fresh and frozen liver homogenates

A

Representative pyruvate + malate seahorse profile using RIFS respirometry protocol in liver homogenates obtained from fresh or frozen tissue (top panel) and quantification (bottom panel).

B

Representative succinate + rotenone seahorse profile using RIFS respirometry protocol in liver homogenates obtained from fresh or frozen tissue (top panel) and quantification (bottom panel).

C

Representative NADH seahorse profile using RIFS respirometry protocol in liver homogenates obtained from fresh or frozen tissue (top panel) and quantification (bottom panel).

D

NADH‐dependent respiration in frozen liver mitochondria in the presence of phenformin and metformin and the indicated concentration.

E

Complex I‐, II‐, and IV‐dependent respiration in liver homogenates from WT and Ndufs4 KO mice showed impaired Complex I with normal Complex II and IV respiration in mouse Ndufs4 KO.

F

Representative Western blot followed by quantification of Complex I (NDUFA9 and NDUFB8) levels in Ndufs4 KO samples. β‐actin was used as loading control.

Data information: Biological replicates: (A and C–F), n = 3–4; (B), n = 4–6. Every biological replicate represents the average of four technical replicates. Data are the mean ± SEM. For calculating statistics, the number of replicates used is the biological replicates indicated above. We used the following tests: two‐way ANOVA with Tukey's multiple comparison test in panels (D and F); unpaired t‐test in panel (E). *P < 0.05; **P < 0.01; ****P < 0.0001.Source data are available online for this figure.

Acknowledgments

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